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Friday, July 31, 2020 | History

2 edition of Towards the development of a fibre-optic nucleic acid biosensor found in the catalog.

Towards the development of a fibre-optic nucleic acid biosensor

James H. Watterson

Towards the development of a fibre-optic nucleic acid biosensor

considerations for the quantitative transduction of hybridization of immobilized DNA.

by James H. Watterson

  • 282 Want to read
  • 25 Currently reading

Published by Dept of Chemistry, U of Toronto .
Written in English


Edition Notes

MICR copy on microfiche (2 microfiches).

ID Numbers
Open LibraryOL19606777M
ISBN 100612459330

  Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost. The feasibility of a biosensor for DNA detection based on suspended-core photonic crystal fibers is investigated. The possibility of functionalization of the hole surface, which allows DNA strand binding, is demonstrated, along with the selective detection of DNA through hybridization of immobilized peptide nucleic acid probes with their full-complementary and mismatched DNA .

2 Development and application of microbiosensors for in-vivo diagnostics + Show details-Hide details p. 21 –35 (15) This review focuses on the advances in development of in-vivo microbiosensors in last five years. Many of the in-vivo microsensors that sometimes are loosely termed “biosensors,”but that don't contain biological recognition elements.   Free Online Library: Nucleic acid diagnostics for fresh water pathogens. (Feature/Chronique).(procedure developed by University of Toronto scientists) by "Canadian Chemical News"; Business Business, international Chemicals, plastics and rubber Biotechnology industries Research Biotechnology industry DNA identification Genetic engineering Medical .

DNA (sometimes referred to as the molecule of life), is the most interesting and most important of all molecules. Electrochemistry of Nucleic Acids and Proteins: Towards Electrochemical Sensors for Genomics and Proteomics is devoted to the electrochemistry of DNA and RNA and to the development of sensors for detecting DNA damage and DNA hybridization. Kwakye S, Baeumner A: A microfluidic biosensor based on nucleic acid sequence recognition. Analytical and Bioanalytical Chemistry , (7) Baeumner AJ, Durst RA: Special Issue - Papers resulting from the 5th Workshop on Biosensors and Biological Techniques in Environmental Analysis Ithaca, NY, USA, 31 May-4th June - Foreword.


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Towards the development of a fibre-optic nucleic acid biosensor by James H. Watterson Download PDF EPUB FB2

TOWARDS THE DEVELOPMENT OF A FIBRE-OPTIC NUCLEIC ACID BIOSENSOR: CONSIDERATIONS FOR THE QUANTITATIVE TRANSDUCTION OF HYBRIDIZATION OF IMMOBILIZED DNA James H.

Watterson A thesis submitted in conformity with the requirements for the Degree of Master of Science, Graduate Department of Cheinistry, at the University of Author: James H. Watterson. Biosensors8 (3), DOI: /bios Zahra Khoshbin, Mohammad Reza Housaindokht, Asma Verdian, Mohammad Reza Bozorgmehr.

Simultaneous detection and determination of mercury (II) and lead (II) ions through the achievement of novel functional nucleic acid-based by:   The relatively poor stability of natural nucleic acids can also limit the practical applications of nucleic acid-based biosensors.

Overcoming the hurdles mentioned above may be achieved through the development and use of highly stable nanomaterials and artificial nucleic acids with superior stability to their natural by: The use of nucleic acid biosensors in a multi-disease configuration for inherited disorders with sufficient mutational homogeneity could allow for early diagnostics for other disorders in addition to SMA.

To this end, this sensor format represents an important step towards the development of biosensors for rapid and quantitative SNP by: A fibre-optic biosensor is described for detection of genomic target sequences from Escherichia coli.A small portion of the LacZ DNA sequence is the basis for selection of DNA probe molecules that are produced by automated nucleic acid synthesis on the surface of optical by:   Biosensors Made of Synthetic Functional Nucleic Acids Toward Better Human Health.

Erin M. McConnell. Advances in functional nucleic acid based paper sensors. Journal of Materials Chemistry B30 DOI: /C9TBG. Partners. DNA biosensors, based on nucleic acid recognition processes, are rapidly being developed towards the assay of rapid, simple and economical testing of genetic and infectious diseases.

Nucleic acids are utilized as a target for development of biosensors as an important tool for disease diagnosis. miRNA (micro-RNA), non-coding and short sequences, regulates the function of mRNAs molecules which are efficient in cellular activity and act as biomarkers for early detection of cancer in plasma, serum and whole blood.

Development of oligonucleotide probe pairs optimized for hybridization at 37°C. In our previous work, the biosensor assays for nucleic acids and protein detection were based on target binding of nucleic acids at 65°C, and protein biomarkers at 37° an effort to integrate the two assays on a single sensor array under uniform target binding conditions (i.e.

37°C), we. Explore the latest full-text research PDFs, articles, conference papers, preprints and more on NUCLEIC ACID CHEMISTRY. Find methods information, sources, references or conduct a literature review.

cells, antibodies, nucleic acids, and biomimetic polymers. Keywords Biosensor Optical sensors Fiber-optic Introduction Biological recognition elements have attracted extraordi-nary interest in recent years, because of the key role they play in the development of highly sensitive and selec-tive chemical analysis.

According to a recently. This book presents a unique concept of merging nanotechnology and novel urinary biomarkers for accurate early prostate cancer detection, discussing an entire progressive pipeline of innovative new str.

The PNA biosensor technology holds promises for rapid and cost-effective detection of specific DNA sequences.

A single-strand nucleic acid probe is immobilised onto optical, electrochemical, or mass-sensitive transducers to detect the complementary (or mismatch) strand in. Nucleic Acids. DNA is an appropriate candidate for biosensing because of its specific ability of base pairing with complementary sequence.

Nucleic acid biosensors (NABs) employ short synthetic single-stranded oligonucleotide probe that is immobilized on the transducer to detect the DNA/RNA in the sample.

These probes can be reused because. For the biosensor to detect the nucleic acid, the virus particles need to be disrupted in a way that releases the nucleic acid from the viral particle.

Disruption of virus particles can be performed via heating or chemical treatment, which adds additional sample preparation procedures into the testing paradigm needed to detect target nucleic acids.

These biosensors exploit hybridization Nucleic acid. between a surface immobilized nucleic acid probe sequence (bioreceptor) and its complementary target sequence.

A probe sequence can be DNA oligomers (ODN) [24] or its modified forms such as peptide nucleic acid (PNA) [25] and locked nucleic acid (LNA) [26]. The complementary sequences. Biosensors Overview The recent trend toward integrated and automated instruments based on bioassays has had an extraordinary impact on the speed and e ciency with which analytical and diagnostic measurements can be made.

Bioas- including nucleic acids, proteins, and fatty acids. Each species serves a. These systems can detect proteins, nucleic acids and pathogens in a few minutes, without the need of fluorescent or colorimetric tags—and with high accuracy.

From plasmonics to silicon photonics For detecting very low amounts of biomarkers, however, the sensitivity of nanoplasmonic biosensors can be limited—or they can require light. In developing the electronic nose and biosensor devices, researchers not only copy biochemical pathways, but also use nature's approach to signal interpretation as a blueprint for man-made sensing systems.

Commercial biosensors have demonstrated their benefits and practical applications, providing high sensitivity and selectivity, combined with a significant reduction in. Histidines incorporated into carriers of nucleic acids may enhance the extracellular stability of the nanoparticle, yet aid in the intracellular disruption of the nanoparticle, enabling the release of the nucleic acid.

Moreover, protonation of histidines in the endosomes may result in endosomal swelling with subsequent lysis. These properties of histidine are based on its five-member. The popular bio-probes that have been employed on biosensor surface for pathogen detection are nucleic acids, antibodies, whole phages, phage-display peptides (PDPs) and most recently phage's receptor binding proteins (RBPs).

Nucleic Acids. The fundamental principle behind nucleic acid based detection lies in the sequence complementarity.Developments over the past few years have revealed the remarkable versatility of RNA in any compartment of the cell, tasks that had been thought to be .Title: The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization VOLUME: 7 ISSUE: 1 Author(s):Hakan Karadeniz, Filiz Kuralay, Serdar Abaci and Arzum Erdem Affiliation:Analytical Chemistry Department, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey.

Keywords:Biosensor, DNA, Nucleic acid hybridization, Electrochemical DNA biosensor.